Human visceral adipose lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 grades C. The human visceral adipose tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the visceral adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The visceral adipose tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Lysate's origin
Tissue
State of tissue
Diseased
Species of the lysate
Human
Cellular origibn of the lysate
Adipose
Disease of the lysate
Diseased
Localisation of the biopsy
Human Visceral Adipose tissue
Ug of lysate used by test
Adipose Visceral lysate is for use in Western blotting, 10 ug to 20 ug per lane is recommended for mini gel.
Positive control in
the lysate is used as positive control in Western Blot
Store at 2-8 grades C for continuous use. For extended storage, freeze working aliquots at -70 grades C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Caution
This product is for research use only.
Laboratory requirements
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. GENTAUR is not liable for damages or injuries resulting from receipt and/or use of GENTAUR materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs).