We do not standard disclose the sequence of our carefully selected and oprimized RT PCR primers.
We will supply you with a microsoft excel template to analyse the qPCR results and see the activation, or supression of genes of interestof your profiler.
Custom 96 or 384 well qPCR primers
1 euro or USD per pig gene added to the qPCR Juvenil Diabetes (Diabetes Type 1) profiling plate.
Roche LightCycler compatibility
The LightCycler 480, for 96 well qPCR: XXXH1-RXS for 384 well: XXXH2-RXS. With perfect Master Mix Sybr Green: PMSX-WXS, 82 Euro
AB cycler RT PCR blocks
The Applied Biosystems Step One Plus Real-Time System 96 well is used with the qPCR XXXH1-FXS with Perfect Master Mix SYBR Green PMSX-RXS, 82 USD and ABI 7900 HT (standard block) XXXH1-AXS, XXXH2-AXS, PMSX-RXS and ViiA7 system (standard block), XXXH1-AXS, XXXH2-AXS, PMSX-LRXS and QuantStudio 6, 7, 12K Flex System (standard block)
Biorad qPCR cyclers
The RT PCR CFX 96 cycler, CFX 384, CFX Connect, Opticon, Chomo, iQ and MyiQ Cycler are compatible withour Perfect Master Mix SYBR Green. Contact us for the reference.
The expression RT PCR profiler array is supplied in 96 and 384 well primer plates. They are used to profile cDNA from human tissues, cells or body fluids. We can perform signaling pathways analysis with only 10-100 cells with the 2018 Direct lysis to SignArray reagent. For rare or precious sampleswe use the SpeAmpn reagents to analyze until 4 signaling pathways with only 5-50ng starting amount of RNA. The adapted hot start SYBR Green qPCR kit apropriate to your Real Time PCR cycler is supplied at 82$ or Euro. Please supply us with your qPCR cyler model when ordering.
Pathway profiling outsourcing
We have a large collection of cell lines and can perfome the Juvenil Diabetes (Diabetes Type 1) expression profiling for you. You can send us your tissues or we have a library of tissues and can perform the RNA extraction, make the cDNA and perform the RT-PCR and qPCR for you.
QPCR Master Mix
We suppy PMS reagent or Perfect Master Mix SYBRG for SignArrays 96 at 10 μl of PMS per reaction for 20 μl final volume. The reference is: PMS1-Z2S, 82 euro